Colt Express takes a new turn with the Marshal & Prisoners expansion. The bandits must face a cunning and powerful Marshal. In fact, the character is now played by one of the players who has their own objectives. They win the game if they fulfill them before the end of the fifth round. A new car occupies the tail of the train: the Prison car. It's the place where you don't want to end up, lest you miss out on fortune... A new arrival is on the train: Mei, the agile climber. She's an accomplished acrobat. The Marshal will have a hard time catching her, and she knows it well.

MARSHAL — Samuel Ford, the Marshal, performs actions different from the bandits. Outlaws will end up in prison if they dare to cross paths with the Marshal on the train; he's like a superhero; he moves and climbs faster than anyone. Tough to be an efficient bandit under these conditions. Additionally, the Marshal has two pistols, and his bullets are specially effective: they disarm, injure, and incapacitate you. The Marshal has two types of objectives: capturing a specific bandit (simple but not easy) and completing a task like shooting all the bandits present on the train or preventing the mail bag from being stolen. Samuel Ford becomes a target as well. Bandits can shoot or hit him. In the latter case, the outlaw gains prestige and the title of Gold, Silver, or Bronze Punch.

PRISONERS — Inside the Prison Car where the Marshal sends the bandits, there are rookie bandits, learning from their elders, the famous bandits of Colt Express. You can release them, and they will assist you by offering their special abilities or by stealing for you.

BRILLANT IDEA — The bandits will be able to perform a new action: the brilliant idea. This will allow them to escape from prison, if they happen to end up there, to free the prisoners inside, but also to mimic the last action played by a bandit.

NEW BANDIT — Mei, the Chinese acrobat. When Mei changes floors, she can move "diagonally" to the floor of an adjacent car.